| Stage 2 (February 22nd): El Roble to Puntarenas ITT, 16 km | ||
| 1 | Inga Cilvinaite (Ltu) Pasta Zara- Cogeas-Manhattan | 23:59 |
| 2 | Lorena Vargas (Col) Iscorp | at 0:37 |
| 3 | Evelyn Garcia (ESa) Pasta Zara- Cogeas-Manhattan | 1:13 |
| 4 | Martina Ruzickova (Cze) Garrobo- Texops | 1:24 |
| 5 | Ana Casas (Mex) Mexican National Team | 1:25 |
| 6 | Addysson Albershardt (USA) Garrobo- Texops | 1:31 |
| 7 | Elena Utrobina (Rus) Liontek | 1:34 |
| 8 | Belen Lopez (Esp) Liontek | 1:42 |
| 9 | Sheyla Gutierrez (Esp) Liontek | 1:45 |
| 10 | Anna Potokina (Rus) Liontek | 1:48 |
| 11 | Katherine Herrera (CRc) Avimil | 2:08 |
| 12 | Agne Silinyte (Ltu) Pasta Zara- Cogeas-Manhattan | 2:11 |
| 13 | Milagro Mena (CRc) Avimil | 2:11 |
| 14 | Veronique Fortin (Can) Garrobo- Texops | 2:13 |
| 15 | Edith Guillen (CRc) Coopeservidores-Giant | 2:34 |
| 16 | Natalia Navarro (CRc) CCDR El Guarco V One V Bic. Focus | 2:46 |
| 17 | Natalia Lopez (CRc) CCDR El Guarco V One V Bic. Focus | 2:58 |
| 18 | Daniela Martinez (CRc) Tostada 1/2 Brooks | 3:02 |
| 19 | Kathyn Bertine (Skn) Team Rayita | 3:05 |
| 20 | Julissa Rios (Pan) Team Rayita | 3:11 |
| 21 | Ingrid Drexel (Mex) Garrobo- Texops | 3:12 |
| 22 | Alexia Muffat (Fra) Liontek | 3:14 |
| 23 | Cassandra Rodriguez (CRc) Avimil | 3:20 |
| 24 | Diana Pe–uela (Col) CCDR Oreamuno | 3:27 |
| 25 | Sylvija Latozaite (Ltu) Garrobo- Texops | 3:36 |
| 26 | Jessica Jurado (Mex) Mexican National Team | 3:41 |
| 27 | Paula Herrera (CRc) CCDR Oreamuno | 3:43 |
| 28 | Rossella Callovi (Ita) Pasta Zara- Cogeas-Manhattan | 3:58 |
| 29 | Jazmin Soto (Gua) Guatemalan National Team | 4:00 |
| 30 | Marcela Rubiano (CRc) Iscorp | 4:11 |
| 31 | Emelyn Galicia (Gua) Guatemalan National Team | 4:25 |
| 32 | Yussely Mendivil (Mex) Mexican National Team | 4:27 |
| 33 | Laura Marin (CRc) CCDR El Guarco V One V Bic. Focus | 4:27 |
| 34 | Edita Janeliute (Ltu) Pasta Zara- Cogeas-Manhattan | 4:34 |
| 35 | Maria Morales (Gua) Guatemalan National Team | 4:39 |
| 36 | Ana Sanabria (Col) Colombian National Team | 4:47 |
| 37 | Fanny Riberot (Fra) Liontek | 4:52 |
| 38 | Gabriela Arias (CRc) Avimil | 4:58 |
| 39 | Cynthia Lee (Gua) Guatemalan National Team | 4:58 |
| 40 | Marcia Rojas (CRc) Team Rayita | 5:23 |
| 41 | Xenia Estrada (Esa) Garrobo- Texops | 5:30 |
| 42 | Ingrid Ramirez (CRc) Team Rayita | 5:33 |
| 43 | Eunice Rojas (CRc) Colombian National Team | 5:34 |
| 44 | Natalia Rojas (CRc) Colombian National Team | 5:52 |
| 45 | Nancy Amores (CRc) CCDR Oreamuno | 5:53 |
| 46 | Valentina Paniagua (Col) Iscorp | 5:58 |
| 47 | Cristina Cortes (CRc) Team Rayita | 6:07 |
| 48 | Ivannia Montero (CRc) CCDR Oreamuno | 6:15 |
| 49 | Adriana Rojas (CRc) Iscorp | 6:20 |
| 50 | Brenda Mu–os (CRc) CCDR El Guarco V One V Bic. Focus | 6:21 |
| 51 | Linnette Gamboa (CRc) Colombian National Team | 6:33 |
| 52 | Susan Camacho (CRc) CCDR Oreamuno | 6:33 |
| 53 | Maria Rojas (CRc) CCDR Oreamuno | 6:37 |
| 54 | Ivania Fonseca (CRc) Tostada 1/2 Brooks | 6:42 |
| 55 | Maira Chinchilla (CRc) CCDR Oreamuno | 6:47 |
| 56 | Kimberly Molina (CRc) Tostada 1/2 Brooks | 7:02 |
| OTL | Giada Borgato (Ita) Pasta Zara- Cogeas-Manhattan | |
| OTL | Karla Piedra (CRc) Team Rayita | |
| OTL | Giddins Hayley (Nz) Iscorp | |
| OTL | Hazel Sancho (CRc) Tostada 1/2 Brooks | |
| OTL | Maria Vicencio (Chi) Tostada 1/2 Brooks | |
| OTL | Yety Aburto (CRc) CCDR Oreamuno | |