| Stage 1 (February 28th): La Libertad to Nahuitalco, 95 km | |
| 1 Noemi Cantele (Ita) Be Pink | 3:04:12 |
| 2 Inga Cilvinaite (Lit) Pasta Zara-Cogeas | at 0:37 |
| 3 Lorena Vargas (Col) Iscorp-Intelligentsia Cofee | 0:39 |
| 4 Clemilda Fernandes (Bra) Team Brazil | 1:23 |
| 5 Serika Guluma (Col) Colombia-Specialized | 1:23 |
| 6 Addyson Albershardt (USA) Garrobo Texops | 1:27 |
| 7 Alena Amialiusik (Blr) Be Pink | 2:18 |
| 8 Ruth Winner (USA) Vanderkitten | 2:18 |
| 9 Kate Chilcott (NZl) Vanderkitten | 2:22 |
| 10 Ana Sanabria (Col) Colombia-Specialized | 2:22 |
| 11 Evelyn Garcia (ESa) Pasta Zara-Cogeas | 2:22 |
| 12 Veronique Fortin (Can) Garrobo Texops | 2:22 |
| 13 Silvija Latozaite (Lit) Lituania-Gatorade | 2:50 |
| 14 Ana Fagua (Col) Colombia-Specialized | 2:58 |
| 15 Lilibeth Chacon (Ven) Team Venezuela | 3:09 |
| 16 Jessica Cutler (USA) Vanderkitten | 3:53 |
| 17 Marcia Fernandes (Bra) Team Brazil | 3:58 |
| 18 Paola Mu–oz (Chi) Iscorp-Intelligentsia Cofee | 4:06 |
| 19 Uenia Souza (Bra) Team Brazil | 4:08 |
| 20 Diana Pe–uela (Col) Colombia-Specialized | 5:22 |
| 21 Marianne Ferreira (Bra) Team Brazil | 5:22 |
| 22 Egle Poskaine (Lit) Lituania-Gatorade | 5:22 |
| 23 Liza Rachetto (USA) Vanderkitten | 5:22 |
| 24 Martina Ruzickova (Cze) Garrobo Texops | 5:22 |
| 25 Doris Schweizer (Sui) Be Pink | 5:36 |
| 26 Ingrid Drexel (Mex) Garrobo Texops | 5:38 |
| 27 Daniely Garcia (Ven) Team Venezuela | 5:38 |
| 28 Milda Jankauskaite (Lit) Lituania-Gatorade | 5:41 |
| 29 Zavnta Titenyte (Lit) Lituania-Gatorade | 5:48 |
| 30 Silvia Valsecchi (Ita) Be Pink | 6:01 |
| 31 Edita Janeliunaite (Lit) Pasta Zara-Cogeas | 6:09 |
| 32 Giada Borgato (Ita) Pasta Zara-Cogeas | 6:09 |
| 33 Dalia Muccioli (Ita) Be Pink | 6:09 |
| 34 Milagro Mena (Cec) Costa Rica | 6:16 |
| 35 Valentina Paniagua (Col) Iscorp-Intelligentsia Cofee | 7:23 |
| 36 Anna Polegatch (Bra) Team Brazil | 7:23 |
| 37 Marisol Tellado (Pur) Iscorp-Intelligentsia Cofee | 7:30 |
| 38 Maria Brice–o (Ven) Team Venezuela | 7:32 |
| 39 Nicole Estrada (Col) Iscorp-Intelligentsia Cofee | 7:48 |
| 40 Susan Camacho (CRc) Costa Rica | 8:52 |
| 41 Rossella Callovi (Ita) Pasta Zara-Cogeas | 9:06 |
| 42 Kathryn Bertine (SKN) Team Rayita | 9:09 |
| 43 Angie Sanabria (Col) Colombia-Specialized | 9:11 |
| 44 Claudia Buitrago (Col) Colombia-Specialized | 9:34 |
| 45 Petra Zrimsek (Slo) Be Pink | 9:44 |
| 46 Agne Silinyte (Lit) Pasta Zara-Cogeas | 9:44 |
| 47 Jurgita Kubiliunaite (Lit) Lituania-Gatorade | 10:16 |
| 48 Angie Gonzalez (Ven) Team Venezuela | 10:29 |
| 49 Staria Teddergreen (USA) Vanderkitten | 11:50 |
| 50 Heylen Guidens (NZl) Iscorp-Intelligentsia Cofee | 11:50 |
| 51 Wilmarys Moreno (Ven) Team Venezuela | 16:49 |
| 52 Xenia Estrada (ESa) Garrobo Texops | 17:48 |
| 53 Ana Gabriela Arias (CRc) Costa Rica | 17:58 |
| 54 Jenni Juhajek (USA) Vanderkitten | 19:50 |
| 55 Jennifer Cesar (Ven) Team Venezuela | 19:50 |
| 56 Paricia Santos (Gua) Iscorp-Intelligentsia Cofe | 28:52 |
| 57 Julissa Rios (Pan) Team Rayita | 32:14 |
| 58 Kristina Norvaisaite (Lit) Lituania-Gatorade | 33:07 |
| 59 Natalia Majano (ESa) Garrobo Texops | 37:32 |
| 60 Cristina Cortes (CRc) Team Rayita | 38:46 |
| 61 Fiorella Rojas (CRc) Team Rayita | 43:48 |
| 62 Maria Natalia Rojas (CRc) Costa Rica | 44:41 |
| 63 Iris Diaz (ESa) Garrobo Texops | 46:10 |
| 64 Juleidy Ramos (Col) Iscorp-Intelligentsia Cofee | 46:48 |