| Stage 4 (March 2nd): Apopa to El Boqueron, 50 km | |
| 1 Noemi Cantele (Ita) Be Pink | 2:05:14 |
| 2 Veronique Fortin (Can) Garrobo Texops | at 0:47 |
| 3 Ana Sanabria (Col) Colombia-Specialized | 0:52 |
| 4 Alena Amialiusik (Blr) Be Pink | |
| 5 Dalia Muccioli (Ita) Be Pink | both s.t. |
| 6 Serika Guluma (Col) Colombia-Specialized | 01:07 |
| 7 Addyson Albershardt (USA) Garrobo Texops | 01:44 |
| 8 Evelyn Garcia (ESa) Pasta Zara-Cogeas | s.t. |
| 9 Doris Schweizer (Sui) Be Pink | 02:00 |
| 10 Clemilda Fernandes (Bra) Team Brazil | 02:29 |
| 11 Lilibeth Chacon (Ven) Team Venezuela | 03:02 |
| 12 Inga Cilvinaite (Lit) Pasta Zara-Cogeas | 03:05 |
| 13 Jessica Cutler (USA) Vanderkitten | s.t. |
| 14 Ana Fagua (Col) Colombia-Specialized | 04:44 |
| 15 Lorena Vargas (Col) Iscorp-Intelligentsia Cofee | 05:15 |
| 16 Ingrid Drexel (Mex) Garrobo Texops | 06:48 |
| 17 Uenia Souza (Bra) Team Brazil | |
| 18 Marcia Fernandes (Bra) Team Brazil | |
| 19 Paola Mu–oz (Chi) Iscorp-Intelligentsia Cofee | all s.t. |
| 20 Martina Ruzickova (Cze) Garrobo Texops | 06:51 |
| 21 Daniely Garcia (Ven) Team Venezuela | s.t. |
| 22 Milagro Mena (Cec) Costa Rica | 07:07 |
| 23 Agne Silinyte (Lit) Pasta Zara-Cogeas | 07:48 |
| 24 Silvia Valsecchi (Ita) Be Pink | 07:59 |
| 25 Rossella Callovi (Ita) Pasta Zara-Cogeas | 08:10 |
| 26 Silvija Latozaite (Lit) Lituania-Gatorade | 08:16 |
| 27 Liza Rachetto (USA) Vanderkitten | 09:45 |
| 28 Ruth Winner (USA) Vanderkitten | 09:45 |
| 29 Edita Janeliunaite (Lit) Pasta Zara-Cogeas | 10:34 |
| 30 Egle Poskaine (Lit) Lituania-Gatorade | 11:04 |
| 31 Milda Jankauskaite (Lit) Lituania-Gatorade | 11:04 |
| 32 Zavnta Titenyte (Lit) Lituania-Gatorade | 11:04 |
| 33 Anna Polegatch (Bra) Team Brazil | 11:37 |
| 34 Staria Teddergreen (USA) Vanderkitten | 11:46 |
| 35 Claudia Buitrago (Col) Colombia-Specialized | 11:53 |
| 36 Marianne Ferreira (Bra) Team Brazil | 11:57 |
| 37 Angie Gonzalez (Ven) Team Venezuela | 11:58 |
| 38 Petra Zrimsek (Slo) Be Pink | 11:58 |
| 39 Xenia Estrada (ESa) Garrobo Texops | 12:14 |
| 40 Susan Camacho (CRc) Costa Rica | 12:33 |
| 41 Giada Borgato (Ita) Pasta Zara-Cogeas | 13:01 |
| 42 Kathryn Bertine (SKN) Team Rayita | 13:36 |
| 43 Marisol Tellado (Pur) Iscorp-Intelligentsia Cofee | 14:22 |
| 44 Maria Brice–o (Ven) Team Venezuela | 14:57 |
| 45 Valentina Paniagua (Col) Iscorp-Intelligentsia Cofee | 15:06 |
| 46 Jennifer Cesar (Ven) Team Venezuela | 15:07 |
| 47 Jurgita Kubiliunaite (Lit) Lituania-Gatorade | 15:09 |
| 48 Ana Gabriela Arias (CRc) Costa Rica | 15:39 |
| 49 Kristina Norvaisaite (Lit) Lituania-Gatorade | 16:50 |
| 50 Diana Pe–uela (Col) Colombia-Specialized | 17:29 |
| 51 Wilmarys Moreno (Ven) Team Venezuela | 18:12 |
| 52 Natalia Majano (ESa) Garrobo Texops | 18:24 |
| 53 Heylen Guidens (NZl) Iscorp-Intelligentsia Cofee | 21:33 |
| 54 Angie Sanabria (Col) Colombia-Specialized | 22:53 |
| 55 Julissa Rios (Pan) Team Rayita | 24:01 |
| 56 Iris Diaz (ESa) Garrobo Texops | 26:21 |
| 57 Paricia Santos (Gua) Iscorp-Intelligentsia Cofe | 28:09 |
| 58 Juleidy Ramos (Col) Iscorp-Intelligentsia Cofee | 32:41 |
| 59 Kate Chilcott (NZl) Vanderkitten | 32:43 |
| 60 Jenni Juhajek (USA) Vanderkitten | 32:43 |
| DNF Fiorella Rojas (CRc) Team Rayita | |
| DNF Maria Natalia Rojas (CRc) Costa Rica |